Sperm Extraction from Absorbed Filter Paper by Shaking Method

Keywords : extraction , shaking method , sperm ,
Publish : December 15, 2012
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Visutr Fongsiripaibul* M.D., LL.M. Sirin Lohacharoensub** M.Sc. Pinnaree Sungkorntanakij* M.Sc.
*Department of Forensic Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
**Department of Clinical Pathology, Faculty of Medicine Vachira Hospital, Bangkok 10400, Thailand

Objective: This study would like to to show an easy and effective way (method) for sperm extraction. The method could be used as basic extraction of sperm from absorbed material; such as swab, cotton or fi lter paper. Materials and

Methods: Filter paper even staining with seminal fl uid was cut (punch) for equal pieces and provided for testing in two major parts, the fi rst part for evaluation of optimal time of extraction by mixer and the second part for comparing between  shaking method and conventional method. The easy way of the experi- ment has been use the fi lter  paper staining with semen and punching in equal pieces, then, the pieces of fi lter papers have been tested step by step for the objective.

Results: The results showed that  the shaking method for extracting sperm from materials such as swab or fi lter paper is signifi cantly better than conventional method (p<0.05). The optimal time for shaking by mixer was three minutes at 1,000 rpm.

Conclusion:  An experimental  sperm extraction from  fi lter paper  stained with  semen  by  shaking method  was  compared  with the conventional  method. The results showed that the shaking method is signifi cantly bet- ter (p<0.05).  The optimal time for shaking was three minutes.  We suggested that the shaking method should be used in routine laboratory in forensic task in Thailand because of the easiness of the test.

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Address of editorial Corresponce
✎  Vorachai Sirikulchayanonta, MD, Editor-in-Chief: Asian Archives of Pathology, Faculty of Science, Rangsit University, Pathumthani 12000, Thailand.
✉  Email address: asianarchpath@gmail.com, vorachai7@gmail.com