Visutr Fongsiripaibul* M.D., LL.M. Sirin Lohacharoensub** M.Sc. Pinnaree Sungkorntanakij* M.Sc. *Department of Forensic Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
**Department of Clinical Pathology, Faculty of Medicine Vachira Hospital, Bangkok 10400, Thailand
Objective: This study would like to to show an easy and effective way (method) for sperm extraction. The method could be used as basic extraction of sperm from absorbed material; such as swab, cotton or ﬁ lter paper. Materials and
Methods: Filter paper even staining with seminal ﬂ uid was cut (punch) for equal pieces and provided for testing in two major parts, the ﬁ rst part for evaluation of optimal time of extraction by mixer and the second part for comparing between shaking method and conventional method. The easy way of the experi- ment has been use the ﬁ lter paper staining with semen and punching in equal pieces, then, the pieces of ﬁ lter papers have been tested step by step for the objective.
Results: The results showed that the shaking method for extracting sperm from materials such as swab or ﬁ lter paper is signiﬁ cantly better than conventional method (p<0.05). The optimal time for shaking by mixer was three minutes at 1,000 rpm.
Conclusion: An experimental sperm extraction from ﬁ lter paper stained with semen by shaking method was compared with the conventional method. The results showed that the shaking method is signiﬁ cantly bet- ter (p<0.05). The optimal time for shaking was three minutes. We suggested that the shaking method should be used in routine laboratory in forensic task in Thailand because of the easiness of the test.