Acute and subchronic oral toxicity of xanthones extracted from the pericarp of Garcinia mangostana Linn.in rat

Publish : September 15, 2015
Total Download : 16

Kanokporn Chayaburakul1, Patamaporn Sukplang2, Thanaporn Pinpart3
and Kanjana Kengkoom4*
1,2Department of Medical Science, Faculty of Science, Rangsit University, Patumthani 12000
1E-mail : kanokporn.c@rsu.ac.th,
3,4Office of Laboratory Animal Production, National Laboratory Animal Center, Mahidol University, Thailand
*Correspondence : Kanjana Kengkoom
4E-mail : directac@mahidol.ac.th
Received : 30th March 2015; Accepted : 23 September 2015

ABSTRACT

The purpose of this research was to study the acute and subchronic toxicity of crude mangosteen pericarp hydro extract, and determine the appropriate and safe amount of extract that may be consumed, through testing with Sprague Dawley rats of both sexes. The soft layer of the inner pericarp mangosteen fruits was immersed in water and boiled at 70 OC, and the supernatant was concentrated and spray-dried into a powder. For the acute
toxicity test, 5 rats of each sex were administered intragastrically with the extract powder dissolving in water at a dose of 2000 mg/kg BW. The animals were then observed closely during the first day post-treatment, before being observed continuously for 14 days. The results of the acute toxicity test showed no abnormalities. For the subchronic toxicity examination, 10 male and 10 female rats were placed in each dosage group for 3 months.
The extract was administered orally at doses of 10, 50, and 100 mg/kg BW/day; distilled water was given to the control group. The extract induced no significant effect on body weight, hematology or the relative weight of organs (brain, heart, lungs, thymus, liver, spleen, kidneys, adrenal glands, testes, and ovaries). Histological examination of the liver, lungs, spleen and heart revealed normal appearance in most all extract treated rat.
Only the kidney of one female rat receiving magosteen pericarp hydro-extract 100 mg/kg BW/day, showed a protruded nucleus in the proximal convoluted tubule. This may be associated with the elevated secretion of creatinine, though creatinine levels remained within reference parameters. Furthermore, the subjects in this group presented torn glomeruli; this may be due to a defect during the histology process, since no white blood
cells or inflammatory cells were identified. The results of this experiment indicated that a safe dosage of the mangosteen pericarp hydro-extract would be < 100 mg/kg BW/day. The effects of subchronic kidney exposure to mangosteen pericarp on kidney require further investigation.

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Address of editorial Corresponce
✎  Vorachai Sirikulchayanonta, MD, Editor-in-Chief: Asian Archives of Pathology, Faculty of Science, Rangsit University, Pathumthani 12000, Thailand.
✉  Email address: asianarchpath@gmail.com, vorachai7@gmail.com