[OA-02] Concordance rate of HER2 immunohistochemistry compared to dual-colour in situ hybridisation (DISH) for detecting HER2 amplification in breast cancer

Gold standard of HER2 amplification detection is fluorescence in situ hybridisation (FISH), but it has several limitations. Many centres use dual-colour in situ hybridisation (DISH) assays to detect HER2 gene amplification, which is cost less and can be done in a general laboratory. ...

Author : Adiluck Pisutpunya, Kroonpong Iampenkhae
Keyword : dual-colour in situ hybridisation, gene amplification, HER2 status, immunohistochemistry
Category : Brief communications

Comparison between unbuffered and buffered formalin fixatives for estrogen receptor and Ki-67 immunostains in breast cancer specimens: a pilot study

Pre-analytic steps are important for the immunohistochemical study as in the case of breast cancers. The initial process is the fixation in which the 10% neutral buffered formalin (NBF) is recommended. 10% formalin is less expensive and easier to prepare but its effect on the immunostaining is controversial....

Author : Chetsada Chunkaruhart, Nopporn Tipparawong, Kroonpong Iampenkhae
Keyword : 10% formalin, 10% neutral buffered formalin, ER, Ki-67, breast cancers
Category : Original Articles