The detection of HLA-B27 allele types by quantitative real-time polymerase chain reaction with melting curve analysis

Human leucocyte antigen B27 (HLA-B27) is a major histocompatibility complex class 1 molecule that is strongly associated with ankylosing spondylitis. The polymerase chain reaction with sequence specific primers (PCR-SSP) is currently used for the discrimination of HLA-B27 allele types resulting from single base substitutions....

Author : Supichaya Nimnuan-ngam, Pasra Arnutti, Thirayost Nimmanon
Keyword : HLA-B27, Real-time PCR, Melting curve analysis
Category : Original Articles